Patrick O Uadia1 
,
Isaac O Imagbovomwan1,
Kelly Oriakhi2,
Ikechi O Eze3
For correspondence:- Patrick Uadia Email: psouadia@uniben.edu Tel:+2348027443746
Accepted: 18 March 2020 Published: 31 August 2020
Citation: Uadia PO, Imagbovomwan IO, Oriakhi K, Eze IO. Effect of Abelmoschus esculentus (okra)-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats. Trop J Pharm Res 2020; 19(8):1737-1743 doi: 10.4314/tjpr.v19i8.24
© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the effects of an okra-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats and its mechanism of action.
Methods: Wistar rats (6) were administered streptozotocin (50 mg/kg ip) after an overnight fast. Upon confirmation of diabetes mellitus, the animals were fed ad libitum for 21 days with formulated okra-based test diet in place of normal diet. The rats treated similarly with streptozotocin and fed ad libitum with the normal diet served as diabetic control while rats fed on normal diet and not treated with streptozotocin served as the negative control. Thereafter, the rats were sacrificed, fasting blood collected and analysed for glucose concentration and biochemical parameters. Pancreas was also excised for histopathological studies.
Results: There was a significant increase in body weight, HDL-cholesterol (p< 0.05) but significant decrease in blood glucose (p < 0.05), serum total cholesterol, triglyceride, LDL-cholesterol and VLDL-cholesterol concentrations in the okra-fed diabetic treated rats when compared to the diabetic control group. Furthermore, superoxide dismutase activity (SOD) was significantly higher in the diabetic control, and reduced significantly when fed with okra-based diet (p < 0.05). Catalase (CAT) activity was significantly (p < 0.05) decreased in diabetic control and treated group, whereas it was significantly (p < 0.05) increased in normal control rats. There was a significant (p < 0.05) decrease in reduced glutathione levels. The significant (p< 0.05) increase in malondialdehyde in the diabetic group was significantly decrease in the diabetic rats fed with okra-diet. Also serum insulin level was significantly (p < 0.05) increased and serum α amylase activity was significantly (p < 0.05) decrease in the diabetic treated rats. Histology results show that there was damage to the β cells of the pancreas in the diabetic control when compared to normal control, but rats fed okra diet was able to regenerate endocrine β cells.
Conclusion: Okra-based diet lowers hyperglycaemia as well as regenerate/repair endocrine β-cells and exocrine tissues of the pancreas damage by streptozotocin.??????
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